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@#ObjectiveTo observe the effect of early rehabilitation on motor function, high-sensitivity C-reactive protein (hs-CRP), interleukin-1（IL-1） and interleukin-6（IL-6） in patients with acute cerebral infarction (ACI). Methods160 patients with ACI were randomly divided into rehabilitation group (n=80) and control group (n=80), who accepted early rehabilitation and routine medicine or medicine only, respectively. They were assessed with modified Edinburgh-Scandinavian Stroke Scale (MESSS) and their peripheral level of hs-CRP, IL-1 and IL-6 were detected before and 1, 2, 4, and 8 weeks after treatment. Results4 and 8 weeks after treatment, the level of hs-CRP, IL-1 and IL-6 were lower in rehabilitation group than in control group（P＜0.05）, as well as the scores of the MESSS （P＜0.01）. The incidence of improvement is more in rehabilitation group than in control group（P＜0.05）. ConclusionEarly rehabilitation can reduce the expression of hs-CRP, IL-1 and IL-6, and improve the neurological function in patients with ACI.
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OBJECTIVE To investigate syphilis serology detection methods suitable for high risk group. METHODS Treponema pallidum(TP-ELISA) and toluidine red un-hoated serum test(TRUST) combined screening methods were taken for sexually transmitted disease(STD) patients and suspicious cases with dirty sexual intercourse,and all the positive results were further confirmed by TPPA method. RESULTS The detection rate of TP-ELISA or TRUST method alons was 18.3% or 13.7%,respectively.The detection rate of the both methods was 20.8%.A percent of 12.0% in 108 cases that were detected positively by the combined screening methods were proved to be false positive by TPPA. CONCLUSIONS The combined screening methods of TP-ELISA and TRUST can reduce the escaping rate,while TPPA can exclude the false positive cases in screening period.Sensitivity and accuracy of syphilis detection can be ensured by taking TP-ELISA and TRUST combined screening with TPPA confirmation methods,which are without high cost and suitable for high risk group.
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BACKGROUND: There are no effective methods to cure Alzheimer disease (AD). Now, researches have shown that panax notoginseng saponins (PNS) play an important role in improving AD, but its mechanism is unclear.OBJECTIVE: To observe the protective effect of PNS characterized by removing blood stasis to stop bleeding and promoting blood circulation to relieve pain on pathological lesion of cholinergic neuron in rat with AD.DESIGN: Completely randomized grouping design and controlled study.SETTING: Neuroscience Institute of Guangxi Traditional Chinese Medical University.MATERIALS: This experiment was completed in the Chinese Herb Pharmacodynamic Laboratory of Guangxi Traditional Chinese Medical University between June 2003 and April 2005. A total of 90 health Wistar rats of clean grade and half gender were selected in this study. Among them, there were 75 old rats with 15 months old and 15 young rats with 3 months old. METHODS: This experiment was completed in the Chinese herb Pharmacodynamic Laboratory (Key Laboratory) of Guangxi Traditional Chinese Medical University between June 2003 and April 2005. ① A total of 90 healthy Wistar rats of clean grade and half gender were selected in this study. Among them, there were 75 old rats with 15 months old and 15 young rats with 3 months old. Fifteen young rats with 3 months old were regarded as young control group, and other 15 selected from 75 rats with 15 months old were regarded as old control group. The rest 60 rats were modeled on the basis of subacute injury induced by intravenous injection of D-galactose and bilateral cerebral Meynert basal nuclei injured by ibotenic acid. Parallel control was performed with saline on rats in young control group and old control group under the same condition. ② Two weeks later,survival modeling rats were divided randomly into 4 groups: model group,high-dosage PNS group, low-dosage PNS group and huperzine A group with 12 in each group. Rats in high-and low-dosage PNS groups were perfused with 200 and 100 mg/kg PNS (provided by Yunnan Yuxi Weihe Pharmaceutical Factory), respectively, once a day; rats in huperzine A group were perfused with 0.3 mg/kg huperzine A once a day for 4 weeks; rats in model group, young control group and old control group were perfused with the same volume of saline for 4 weeks. ③ After administration, pathological sections of brain tissue were cut, and immunologic-reaction activity of choline acetyltransferase (ChAT), morphological changes and numbers of positive neuron in cerebral sections were determined by immunohistochemistry analysis. ChAT immuno-positive neurons were analyzed with IBAS imaging analysis system to assay average area of section and average absorbance (A), and amount of ChAT immuno-positive neurons was calculated with microscope micrometer. ④ Measurement data were compared with single-factor analysis of variance.MAIN OUTCOME MEASURES: Effect of PNS on distribution of cholinergic neuron and ChAT content in cerebral tissue of AD rat models.RESULTS: A total of 75 old rats and 15 young rats entered the final analysis. ① Amount of ChAT immuno-positive neurons was the most, and the color was the deepest in young control group; amount of ChAT immuno-positive neurons was higher in high-dosage PNS group than that in huperzine A group and model group; ChAT immuno-positive neurons were smaller in model group than those in other goups, and the amount was decreased obviously. Axis-cylinder and dendrite of soma were shortened remarkably. ② Amounts of ChAT immuno-positive neurons in basal forebrain were less in model group than those in other groups (P ＜ 0.05), less in lowdosage PNS group, huperzine A group and model group than those in old control group (P ＜ 0.05), less in huperzine A group and model group than those in high- and low-dosage PNS group (P ＜ 0.05), and less in young control group than those in other groups (P ＜ 0.05). The mean A value of ChAT immuno-positive neurons in basal forebrain was similar to amounts in each group. Average area of section of ChAT immuno-positive neurons in basal forebrain was smaller in low-dosage PNS group and model group than that in young control group (P ＜ 0.05), and differences in other groups were not significant (P ＞ 0.05).CONCLUSION: PNS plays a protective role in pathological lesion of cholinergic neuron in AD rat models. PNS can also increase survival amount and quality of cell and increase content and activity of ChAT so as to protect and improve central cholinergic system, and inhibit aging and dementia through improving and repairing injured cholinergic neurons.
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Objective To evaluate the efficacy of free-radical scavenger in the treatment of chronic prostatitis (CP). Methods 117 patients with CP were randomly divided into 4 groups: group A (32 patients) were treated with free-radical scavenger, group B (32 patients) with antibiotics, group C (32 patients) with ?-blocker and group D with placebo. Microscopic examination,bacterial culture,SOD and MDA examination were conducted just before and after the procedure. Results No cure was observed in each group.18 patients were improved in group A,15 in group B,21 in group C and 2 in group D. The efficacy rate was 47.3%,46.8%,65.6% and 13.3% respectively. Compared to the placebo group, the free-radical scavenger had a significant therapeutic efficacy and had no significant difference to the antibiotic group and ?-blocker group. Conclusions The free-radical scavenger may be a useful method for the treatment of chronic prostatitis.
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[Objective] To observe the anti-aging actions of total saponins of Radix Notoginseng, i.e., Panax Notoginseng saponins (PNS) on rats with Alzheimer's Disease (AD). [Methods] Among 90 Wistar rats, 15 rats aged 3 months were allocated to the youth group, 15 aged 15 months to the aged group, and other 60 aged 15 months were given with intraperitoneal injection of D-galactose and injection of ibotenic acid into bilateral Meynert nucleus basalis to establish the models of AD. After then, the surviving model rats were randomized into four groups: model control, high-dose PNS (200mg?kg-1?d-1), low-dose PNS (100 mg?kg-1?d-1) and huperzine A (0.3 mg?kg-1?d-1). Except the model group, the youth group and the aged group were given the same amount of normal saline by gavage, the other groups were treated with the designed drugs respectively for 4 weeks. After treatment, serum levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), triglyceride (TG) and cholesterol were detected. [Results] High- and low-dose PNS increased the serum levels of SOD, GSH and CAT, the differences being significance as compared with the model group (P